mouse TOP1MT Over-expression AAV

Name 
AAV-m-TOP1MT
Cat No 
AAV-274815
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Mouse Top1mt.

AAV-274815
AAV-m-TOP1MT

Ready-to-use AAV expressing Mouse Top1mt (DNA topoisomerase 1, mitochondrial). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2900052H09Rik
Description (Vector)
Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Gene ID
72960
Gene Name (Vector)
DNA topoisomerase 1, mitochondrial
Gene Symbol
Top1mt
ORF Size (aa)
1779
ORF Size (bp)
1779 bp
RefSeq ID
NM_028404
RefSeq Synonyms
NM_028404, BC145336, BC138583, BC138582,
Species
mouse
UniGene ID
Mm.182401

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.