mouse SHARPIN Over-expression AAV
This is an AAV expressing Mouse Sharpin.
AAV-271931
AAV-m-SHARPIN
Ready-to-use AAV expressing Mouse Sharpin (SHANK-associated RH domain interacting protein). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
0610041B22Rik; AW121341; cpdm; RBCKL1; SIPL1
Description (Vector)
Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex.
Gene ID
106025
Gene Name (Vector)
SHANK-associated RH domain interacting protein
Gene Symbol
Sharpin
ORF Size (aa)
918
ORF Size (bp)
918 bp
RefSeq ID
NM_025340
RefSeq Synonyms
NM_025340, BC055758, BC016203,
Species
mouse
UniGene ID
Mm.41463
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.