mouse AOF1 Over-expression AAV

Name 
AAV-m-AOF1
Cat No 
AAV-252754
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Mouse Kdm1b.

AAV-252754
AAV-m-AOF1

Ready-to-use AAV expressing Mouse Kdm1b (lysine (K)-specific demethylase 1B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
4632428N09Rik; AI482520; Aof1
Description (Vector)
Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (By similarity).
Gene ID
218214
Gene Name (Vector)
lysine (K)-specific demethylase 1B
Gene Symbol
Kdm1b
ORF Size (aa)
2481
ORF Size (bp)
2481 bp
RefSeq ID
NM_172262
RefSeq Synonyms
NM_172262, BC023917,
Species
mouse
UniGene ID
Mm.31259

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.