human DGCR8 Over-expression AAV

Name 
AAV-h-DGCR8
Cat No 
AAV-232656
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human DGCR8.

AAV-232656
AAV-h-DGCR8

Ready-to-use AAV expressing Human DGCR8 (DGCR8, microprocessor complex subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
C22orf12; DGCRK6; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Gy1; microprocessor complex subunit DGCR8; pasha
Description (eCommerce)
DiGeorge syndrome critical region 8 (DGCR8) is a component of the microprocessor complex that acts as a RNA- and heme-binding protein and is involved in the initial step of microRNA (miRNA) biogenesis. DGCR8 functions as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junctions. It directs RNASEN/DROSHA to cleave 11 bp away from the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8WYQ5].
Description (Vector)
DGCR8 encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.
Gene ID
54487
Gene Name (eCommerce)
DGCR8, microprocessor complex subunit
Gene Name (Vector)
DGCR8, microprocessor complex subunit
Gene Symbol
DGCR8
HGNC ID
HGNC:2847
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2223
ORF Size (bp)
2223 bp
Protein Name (eCommerce)
Microprocessor complex subunit DGCR8
RefSeq ID
NM_022720
RefSeq Synonyms
NM_022720, NM_001190326, BC078147, BC009323,
Research Areas
Cytoskeleton,Gene Expression,MicroRNAs in Cancer,RNA Binding,RNA Processing
Research Areas (Faceted)
cancer,genetics,cell_biology,genetics,transcription_translation
Species
human
Target Sentence
DiGeorge syndrome critical region 8 (DGCR8) is a component of the microprocessor complex that acts as a RNA- and heme-binding protein and is involved in the initial step of microRNA (miRNA) biogenesis. DGCR8 functions as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junctions. It directs RNASEN/DROSHA to cleave 11 bp away from the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8WYQ5].
UniGene ID
Hs.643452
UniProt ID (eCommerce)
Q8WYQ5

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.