human TFEB Over-expression AAV

Name 
AAV-h-TFEB
Cat No 
AAV-225358
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human TFEB.

AAV-225358
AAV-h-TFEB

Ready-to-use AAV expressing Human TFEB (transcription factor EB). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ALPHATFEB; BHLHE35; class E basic helix-loop-helix protein 35; T-cell transcription factor EB; TCFEB; transcription factor EB
Description (eCommerce)
Transcription factor EB (TFEB) specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, TFEB activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression; thus, TFEB plays a central role in expression of lysosomal genes. TFEB also specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P19484].
Gene ID
7942
Gene Name (eCommerce)
transcription factor EB
Gene Name (Vector)
transcription factor EB
Gene Symbol
TFEB
HGNC ID
HGNC:11753
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1431
ORF Size (bp)
1431 bp
Protein Name (eCommerce)
Transcription factor EB
RefSeq ID
NM_007162
RefSeq Synonyms
NM_007162, NM_001271945, NM_001271944, NM_001271943, NM_001167827, BC032448,
Research Areas
Autophagy,Immunology,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,immunology,transcription_translation
Species
human
Target Sentence
Transcription factor EB (TFEB) specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, TFEB activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression; thus, TFEB plays a central role in expression of lysosomal genes. TFEB also specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P19484].
UniGene ID
Hs.715310
UniProt ID (eCommerce)
P19484

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.