human SPHK1 Over-expression AAV

Name
AAV-h-SPHK1

Cat No
AAV-224165

Availability
4-5 weeks

This is an AAV expressing Human SPHK1.

AAV-224165

AAV-h-SPHK1

Ready-to-use AAV expressing Human SPHK1 (sphingosine kinase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

CMV (default), or choose from 30 different ubiquitous or cell specific promoter

Reporter

none, optional GFP, CFP, YFP, RFP or mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

SK 1; sphingosine kinase 1; SPHK; SPK 1

Description (eCommerce)

Sphingosine kinase 1 (SPHK1) catalyzes the phosphorylation of sphingosine to yield sphingosine-1-phosphate (SPP). SPP functions as a lipid signaling molecule that mediates both intra- and extra-cellular responses. SPHK1activity may play a role in several cellular processes such as apoptisis, proliferation, inflammation, and migration.

Description (Vector)

SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene ID

8877

Gene Name (eCommerce)

sphingosine kinase 1

Gene Name (Vector)

sphingosine kinase 1

Gene Symbol

SPHK1

HGNC ID

HGNC:11240

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1413

ORF Size (bp)

1413 bp

Protein Name (eCommerce)

Sphingosine kinase 1

RefSeq ID

NM_182965

RefSeq Synonyms

NM_182965, NM_021972, NM_001142602, NM_001142601, BC030553, BC014439, BC009419, BC008040,

Research Areas

Angiogenesis,Apoptosis,Brain Development,Cancer,Cell Cycle,Fc Receptor Signaling,Immunology,Kinase/Phosphatase,Metabolism/Metabolic Process,Neurobiology,Neurodevelopment,PDGF Signaling,Protein Folding,Signal Transduction,Translational Control,VEGF Signaling

Research Areas (Faceted)

cancer,cell_biology,cell_cycle,developmental_biology,genetics,immunology,metabolism,neurobiology,signal_transduction,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.68061

UniProt ID (eCommerce)

Q9NYA1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.