human PPM1D Over-expression AAV

Name
AAV-h-PPM1D

Cat No
AAV-219575

Availability
4-5 weeks

This is an AAV expressing Human PPM1D.

AAV-219575

AAV-h-PPM1D

Ready-to-use AAV expressing Human PPM1D (protein phosphatase, Mg2+/Mn2+ dependent 1D). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

CMV (default), or choose from 30 different ubiquitous or cell specific promoter

Reporter

none, optional GFP, CFP, YFP, RFP or mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

PP2C-DELTA; WIP1

Description (Vector)

PPM1D is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

Gene ID

8493

Gene Name (Vector)

protein phosphatase, Mg2+/Mn2+ dependent 1D

Gene Symbol

PPM1D

HGNC ID

HGNC:9277

ORF Size (aa)

1818

ORF Size (bp)

1818 bp

RefSeq ID

NM_003620

RefSeq Synonyms

NM_003620, BC060877, BC042418, BC033893, BC016480,

Species

human

UniGene ID

Hs.591184

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.