human PAFAH1B1 Over-expression AAV

Name 
AAV-h-PAFAH1B1
Cat No 
AAV-218127
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human PAFAH1B1.

AAV-218127
AAV-h-PAFAH1B1

Ready-to-use AAV expressing Human PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
LIS1; LIS-1; LIS2; lissencephaly 1 protein; Lissencephaly-1 protein; MDCR; MDS; NudF; PAF acetylhydrolase 45 kDa subunit; PAFAH; PAF-AH 45 kDa subunit; PAFAH alpha; PAF-AH alpha; platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa); platelet-activating factor acetylhydrolase IB subunit alpha; platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD); platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)
Description (eCommerce)
Lissencephaly-1 protein (LIS-1) was identified as a gene associated with Miller-Dieker lissencephaly syndrome, an autosomal dominant disorder characterized by microcephaly along with anomalies of the kidney, heart, and gastrointestinal tract. LIS-1 is the non-catalytic alpha subunit of the intracellular isoform of platelet-activating factor acetylhydrolase. LIS-1 plays an important role in neuron migration during brain development and fibroblast migration during wound healing. Its role in migration is related to its function in regulating dynein-mediated microtubule sliding.
Description (Vector)
This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.
Gene ID
5048
Gene Name (eCommerce)
platelet activating factor acetylhydrolase 1b regulatory subunit 1
Gene Name (Vector)
platelet activating factor acetylhydrolase 1b regulatory subunit 1
Gene Symbol
PAFAH1B1
HGNC ID
HGNC:8574
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1233
Protein Name (eCommerce)
Platelet-activating factor acetylhydrolase IB subunit alpha
RefSeq ID
NM_000430
RefSeq Synonyms
NM_000430, BC064638,
Research Areas
Cell Cycle,Cell Division,Cytoskeleton,Neurobiology,Neurodevelopment,Neurogenesis,Signal Transduction,Transport,Wnt Signaling
Research Areas (Faceted)
cell_biology,cell_cycle,developmental_biology,genetics,neurobiology,signal_transduction
Species
human
Target Sentence
Lissencephaly-1 protein (LIS-1) was identified as a gene associated with Miller-Dieker lissencephaly syndrome, an autosomal dominant disorder characterized by microcephaly along with anomalies of the kidney, heart, and gastrointestinal tract. LIS-1 is the non-catalytic alpha subunit of the intracellular isoform of platelet-activating factor acetylhydrolase. LIS-1 plays an important role in neuron migration during brain development and fibroblast migration during wound healing. Its role in migration is related to its function in regulating dynein-mediated microtubule sliding.
UniGene ID
Hs.77318
UniProt ID (eCommerce)
P43034

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.