human P4HB Over-expression AAV

Name 
AAV-h-P4HB
Cat No 
AAV-218080
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human P4HB.

AAV-218080
AAV-h-P4HB

Ready-to-use AAV expressing Human P4HB (prolyl 4-hydroxylase subunit beta). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
cellular thyroid hormone-binding protein; CLCRP1; collagen prolyl 4-hydroxylase beta; DSI; ERBA2L; GIT; glutathione-insulin transhydrogenase; P4Hbeta; p55; PDI; PDIA1; PHDB; PO4DB; PO4HB; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; PROHB; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase family A, member 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase-associated 1; protein disulfide-isomerase; protocollagen hydroxylase; testicular secretory protein Li 32; thyroid hormone-binding protein p55
Description (eCommerce)
P4HB is a multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, P4HB seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P07237].
Description (Vector)
P4HB encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
Gene ID
5034
Gene Name (eCommerce)
prolyl 4-hydroxylase subunit beta
Gene Name (Vector)
prolyl 4-hydroxylase subunit beta
Gene Symbol
P4HB
HGNC ID
HGNC:8548
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1527
ORF Size (bp)
1527 bp
Protein Name (eCommerce)
Protein disulfide-isomerase
RefSeq ID
NM_000918
RefSeq Synonyms
NM_000918, BC071892, BC029617, BC010859,
Research Areas
Golgi Apparatus,Hypoxia,Metabolism/Metabolic Process,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology,metabolism
Species
human
Target Sentence
P4HB is a multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, P4HB seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P07237].
UniGene ID
Hs.464336
UniProt ID (eCommerce)
P07237

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.