human NFYA Over-expression AAV

Name 
AAV-h-NFYA
Cat No 
AAV-216664
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human NFYA.

AAV-216664
AAV-h-NFYA

Ready-to-use AAV expressing Human NFYA (nuclear transcription factor Y subunit alpha). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; CBF-A; CBF-B; CCAAT-binding transcription factor subunit B; HAP2; HAP2 CCAAT-binding protein; NF-YA; nuclear transcription factor Y subunit A; nuclear transcription factor Y subunit alpha; nuclear transcription factor Y, alpha; Transcription factor NF-Y, A subunit
Description (eCommerce)
Nuclear transcription factor Y alpha (NF-YA) is a subunit of the NF-Y heterotrimeric complex composed of NF-YA, NF-YB, and NF-YC. The NF-Y factor binds the CCAAT box with high specificity and affinity, and it is the major CCAAT-binding activator. All 3 subunits are essential for CCAAT binding. NF-Y plays a role in regulating several cell-cycle regulated genes such as CDC2, CDC25A/B/C, cyclin A2, cyclin B1/B2, and E2F1.
Description (Vector)
NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
Gene ID
4800
Gene Name (eCommerce)
nuclear transcription factor Y subunit alpha
Gene Name (Vector)
nuclear transcription factor Y subunit alpha
Gene Symbol
NFYA
HGNC ID
HGNC:7804
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
957
ORF Size (bp)
957 bp
Protein Name (eCommerce)
Nuclear transcription factor Y subunit alpha
RefSeq ID
NM_002505
RefSeq Synonyms
NM_021705, NM_002505, BC039244,
Research Areas
Antigen Processing and Presentation,Immunology,Metabolism/Metabolic Process,Protein Folding,Signal Transduction,TGF-beta Signaling,Transcription Factor/Regulator,Wnt Signaling
Research Areas (Faceted)
cell_biology,immunology,metabolism,signal_transduction,transcription_translation
Species
human
Target Sentence
Nuclear transcription factor Y alpha (NF-YA) is a subunit of the NF-Y heterotrimeric complex composed of NF-YA, NF-YB, and NF-YC. The NF-Y factor binds the CCAAT box with high specificity and affinity, and it is the major CCAAT-binding activator. All 3 subunits are essential for CCAAT binding. NF-Y plays a role in regulating several cell-cycle regulated genes such as CDC2, CDC25A/B/C, cyclin A2, cyclin B1/B2, and E2F1.
UniGene ID
Hs.10441
UniProt ID (eCommerce)
P23511

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.