human MKL1 Over-expression AAV

Name 
AAV-h-MKL1
Cat No 
AAV-215498
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human MKL1.

AAV-215498
AAV-h-MKL1

Ready-to-use AAV expressing Human MKL1 (megakaryoblastic leukemia (translocation) 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
basic, SAP and coiled-coil domain; BSAC; MAL; megakaryoblastic leukemia (translocation) 1; megakaryoblastic leukemia 1 protein; megakaryocytic acute leukemia protein; MKL; MKL/myocardin-like protein 1; MKL1; MRTF-A
Description (eCommerce)
MKL1 (megakaryoblastic leukemia 1 protein) was identified as part of a t(1;22) translocation with RBM15 associated with acute megakaryoblastic leukemia. It is a transcriptional coactivator of serum response factor (SRF) that promotes muscle differentiation and also plays a role in promoting maturation of megakaryocytes.
Description (Vector)
MKL1 interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia.
Gene ID
57591
Gene Name (eCommerce)
myocardin related transcription factor A
Gene Name (Vector)
megakaryoblastic leukemia (translocation) 1
Gene Symbol
MKL1
HGNC ID
HGNC:14334
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2793
ORF Size (bp)
2793 bp
Protein Name (eCommerce)
MKL/myocardin-like protein 1
RefSeq ID
NM_020831
RefSeq Synonyms
NM_020831, NM_001318139, NM_001282662, NM_001282661, NM_001282660, BC141655, BC141653, BC115039,
Research Areas
Cancer,Cell Biology,Cytoskeleton,Rho Signaling,Signal Transduction,Transcription Factor/Regulator
Research Areas (Faceted)
cancer,cell_biology,signal_transduction,transcription_translation
Species
human
Target Sentence
MKL1 (megakaryoblastic leukemia 1 protein) was identified as part of a t(1;22) translocation with RBM15 associated with acute megakaryoblastic leukemia. It is a transcriptional coactivator of serum response factor (SRF) that promotes muscle differentiation and also plays a role in promoting maturation of megakaryocytes.
UniGene ID
Hs.654688
UniProt ID (eCommerce)
Q969V6

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.