human MGEA5 Over-expression AAV

Name 
AAV-h-MGEA5
Cat No 
AAV-215423
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human MGEA5.

AAV-215423
AAV-h-MGEA5

Ready-to-use AAV expressing Human MGEA5 (meningioma expressed antigen 5 (hyaluronidase)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
beta-hexosaminidase; beta-N-acetylglucosaminidase; beta-N-acetylhexosaminidase; bifunctional protein NCOAT; hyaluronidase in meningioma; MEA5; meningioma expressed antigen 5 (hyaluronidase); meningioma-expressed antigen 5; MGEA5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; NCOAT; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; OGA; protein O-GlcNAcase
Description (eCommerce)
Meningioma-expressed antigen 5 (MGEA5) cleaves GlcNAc but not GalNAc from glycopeptides. MGEA5 can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc.MGEA5 possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60502].
Description (Vector)
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 (PubMed 11148210)).(supplied by OMIM, Mar 2008)
Gene ID
10724
Gene Name (eCommerce)
O-GlcNAcase
Gene Name (Vector)
meningioma expressed antigen 5 (hyaluronidase)
Gene Symbol
MGEA5
HGNC ID
HGNC:7056
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2550
ORF Size (bp)
2550 bp
Protein Name (eCommerce)
Protein O-GlcNAcase
RefSeq ID
NM_012215
RefSeq Synonyms
NM_012215, NM_001142434, BC039583,
Research Areas
Acyltransferase,Aging,Apoptosis,Cardiology,Cell Biology,Insulin Signaling,Metabolism/Metabolic Process,Mitochondrion,Transport
Research Areas (Faceted)
cardiovascular,cell_biology,metabolism,signal_transduction
Species
human
Target Sentence
Meningioma-expressed antigen 5 (MGEA5) cleaves GlcNAc but not GalNAc from glycopeptides. MGEA5 can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc.MGEA5 possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60502].
UniGene ID
Hs.500842
UniProt ID (eCommerce)
O60502

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.