human ASAP2 Over-expression AAV
This is an AAV expressing Human ASAP2.
AAV-201510
AAV-h-ASAP2
Ready-to-use AAV expressing Human ASAP2 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AMAP2; arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2; centaurin, beta 3; CENTB3; DDEF2; development and differentiation-enhancing factor 2; PAG3; PAP; Pap-alpha; paxillin-associated protein with ARF GAP activity 3; PYK2 C terminus-associated protein; pyk2 C-terminus-associated protein; SHAG1
Description (eCommerce)
ASAP2 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 2) activates the small GTPases ARF1, ARF5 and ARF6. It regulates the formation of post-Golgi vesicles and modulates constitutive secretion. ASAP2 also modulates phagocytosis mediated by Fc gamma receptor and ARF6 as well as PXN recruitment to focal contacts and cell migration [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ O43150].
Description (Vector)
ASAP2 encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
8853
Gene Name (eCommerce)
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Gene Name (Vector)
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Gene Symbol
ASAP2
HGNC ID
HGNC:2721
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2886
ORF Size (bp)
2886 bp
Protein Name (eCommerce)
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
RefSeq ID
NM_003887
RefSeq Synonyms
NM_003887, NM_001135191, BC063308,
Research Areas
Golgi Apparatus,Immunology,RAC1 Signaling,Rho Signaling,Signal Transduction,T-cell Receptor Signaling
Research Areas (Faceted)
signal_transduction,cell_biology,immunology,signal_transduction
Species
human
Target Sentence
ASAP2 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 2) activates the small GTPases ARF1, ARF5 and ARF6. It regulates the formation of post-Golgi vesicles and modulates constitutive secretion. ASAP2 also modulates phagocytosis mediated by Fc gamma receptor and ARF6 as well as PXN recruitment to focal contacts and cell migration [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ O43150].
UniGene ID
Hs.555902
UniProt ID (eCommerce)
O43150
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.