human ASAH2 Over-expression AAV

Name
AAV-h-ASAH2

Cat No
AAV-201507

Availability
4-5 weeks

This is an AAV expressing Human ASAH2.

AAV-201507

AAV-h-ASAH2

Ready-to-use AAV expressing Human ASAH2 (N-acylsphingosine amidohydrolase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

CMV (default), or choose from 30 different ubiquitous or cell specific promoter

Reporter

none, optional GFP, CFP, YFP, RFP or mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

BCDase; HNAC1; LCDase; N-CDase; NCDase

Description (Vector)

Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 (PubMed 11328816)).(supplied by OMIM, Mar 2008)

Gene ID

56624

Gene Name (Vector)

N-acylsphingosine amidohydrolase 2

Gene Symbol

ASAH2

HGNC ID

HGNC:18860

ORF Size (aa)

2181

ORF Size (bp)

2181 bp

RefSeq ID

NM_019893

RefSeq Synonyms

NM_019893, NM_001143974, BC107105,

Species

human

UniGene ID

Hs.512645

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.