human AFF4 Over-expression AAV

Name 
AAV-h-AFF4
Cat No 
AAV-200534
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human AFF4.

AAV-200534
AAV-h-AFF4

Ready-to-use AAV expressing Human AFF4 (AF4/FMR2 family member 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AF4/FMR2 family member 4; AF5Q31; ALL1-fused gene from chromosome 5q31 protein; CHOPS; major CDK9 elongation factor-associated protein; MCEF; Protein AF-5q31
Description (eCommerce)
Major CDK9 elongation factor-associated protein (MCEF) is also known as AFF4 AF4/FMR2 family member 4. This family of proteins includes the AF4 proto-oncogene and FMR2 (fragile X E mental retardation syndrome) nuclear proteins. MCEF is a component of the cyclin-dependent kinase pair (CDK9/cyclin T1) complex. A chromosomal aberration involving AFF4 is found in acute lymphoblastic leukemia (ALL).
Description (Vector)
AFF4 belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23).
Gene ID
27125
Gene Name (eCommerce)
AF4/FMR2 family member 4
Gene Name (Vector)
AF4/FMR2 family member 4
Gene Symbol
AFF4
HGNC ID
HGNC:17869
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3492
ORF Size (bp)
3492 bp
Protein Name (eCommerce)
AF4/FMR2 family member 4
RefSeq ID
NM_014423
RefSeq Synonyms
NM_014423, BC137226, BC063007,
Research Areas
Cancer,Mitochondrion,Transcription Factor/Regulator
Research Areas (Faceted)
cancer,cell_biology,transcription_translation
Species
human
Target Sentence
Major CDK9 elongation factor-associated protein (MCEF) is also known as AFF4 AF4/FMR2 family member 4. This family of proteins includes the AF4 proto-oncogene and FMR2 (fragile X E mental retardation syndrome) nuclear proteins. MCEF is a component of the cyclin-dependent kinase pair (CDK9/cyclin T1) complex. A chromosomal aberration involving AFF4 is found in acute lymphoblastic leukemia (ALL).
UniGene ID
Hs.664840
UniProt ID (eCommerce)
Q9UHB7

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.