AAV-Cre (AAV serotype 5) AAV
This AAV serotype 5 virus (Capsid from AAV5 and ITR from AAV2) expresses Cre Recombinase under the control of a CMV promoter. This Cre contains a nuclear localization signal (NLS).
Cre recombinase is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cultured cells or experimental animals.
7012
AAV5-Cre
Ready-to-use AAV-Cre (AAV serotype 5). AAV2/5-Cre AAV5-Cre Cre recombinase AAV serotype 5 7012
Related Citations
- Visualization and correction of social abnormalities-associated neural ensembles in adult MECP2 duplication mice. L Sun, etc, (2020), Science Bulletin
- The amygdala instructs insular feedback for affective learning. D Kargl, etc, (2020), eLIFE
- Polycomb repressive complex 2 (PRC2) silences genes responsible for neurodegeneration. von Schimmelmann M, etc, (2016), Nat Neurosci
- eIF2a-mediated translational control regulates the persistence of cocaine-induced LTP in midbrain dopamine neurons. Placzek AN, etc, (2016), eLife
- Translational control by eIF2a phosphorylation regulates vulnerability to the synaptic and behavioral effects of cocaine. W Huang, etc, (2016), eLife
- Control of Stress-Induced Persistent Anxiety by an Extra-Amygdala Septohypothalamic Circuit. Anthony, TE. etc, (2014), Cell
- LKB1 and AMPK regulate synaptic remodeling in old age. Melanie A Samuel, etc, (2014), Nature Neuroscience
- Translational control of mGluR-dependent long-term depression and object-place learning by eIF2a. Gonzalo Viana Di Prisco, etc, (2014), Nature Neuroscience
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.