Myogenic differentiation 1 Adenovirus

Name 
Ad-MyoD
Cat No 
1492
Availability
Immediate

Differentiation of myogenic cells is regulated by multiple positive and negative regulators. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes MyoD, myogenin, Myf-5, Myf-6 (also designated MRF-4 or herculin) and musculin (also designated MyoR). Members of this group of transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, ITF2 and HEB. The E2A gene encodes at lest two different proteins, E12 and E47, as a result of alternative splicing. The myocyte enhancing factor-2 (MEF-2) family of transcription factors includes four splice variants A, B, C and D which encode muscle-specific DNA binding proteins that recognize an A/T rich sequence [CTA (A/T)4 TAG] localized in the control regions of numerous muscle-specific genes. This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.

1492
Ad-MyoD

Ready-to-use Myogenic differentiation 1 Adenovirus. Ad-MyoD, Myogenic differentiation 1, PUM,MYF3,MYOD,MYOD1 adenovirus 1492

$475.00
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Product Details

Promoter
CMV (ubiquitous)
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
bHLHc1; MYF3; MYOD; PUM
Description (Vector)
MYOD1 encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Gene ID
4654
Gene Name (Vector)
myogenic differentiation 1
Gene Symbol
MYOD1
HGNC ID
HGNC:7611
ORF Size (aa)
963
ORF Size (bp)
963 bp
RefSeq ID
NM_002478
RefSeq Synonyms
NM_002478, BC064493,
Species
human
UniGene ID
Hs.181768

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.