RAS p21 protein activator (GTPase activating protein) 1 Adenovirus

Name: RAS p21 protein activator (GTPase activating protein) 1 Adenovirus
SKU: 1395
Availability: InStock

Name
Ad-RasGAP

Cat No
1395

Availability
Immediate

Ras p21 can exist in either a physiologically quiescent GDP-binding state or a GTP-binding signal-emitting state. Interaction of Ras p21 with GTPase activating protein (GAP) can increase the rate of hydrolysis of Ras p21-bound GTP by as much as 1000-fold. In mitogenically activated and tyrosine kinase-transformed cells, Ras GAP forms a complex with a protein designated p190. At its N-terminus, p190 contains sequence motifs characteristic of all known GTPases, whereas the C-terminus contains sequences similar to those found in the Bcr gene product, n-chimerin and Rho GAP, all of which exhibit intrinsic GAP activity. Additional proteins with GTPase activating activity include GAP-associated protein p190 and Ral BP-1 (also designated RLIP 76), which displays a GAP activity specific for Rac 1 and Cdc42, Gap1m and oligophrenin-1. Oligophrenin-1 is a RhoGAP protein that stimulates GTP hydrolysis of Rho subfamily members and is involved in cell migration, morphogenesis and axon outgrowth.

1395

Ad-RasGAP

Ready-to-use RAS p21 protein activator (GTPase activating protein) 1 Adenovirus. Ad-RasGAP, RAS p21 protein activator (GTPase activating protein) 1, GAP,PKWS,RASA,CMAVM,RASA1,RASGAP,P120GAP adenovirus 1395

$690.00

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Product Details

Promoter

CMV (ubiquitous)

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Titer

1x10^10 PFU/ml

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Volume

200 µL

Gene Reference Data

Alternate Names

CMAVM; CM-AVM; GAP; GTPase-activating protein; p120; p120 RAS GTPase activating protein; p120GAP; p120RASGAP; PKWS; ras GTPase-activating protein 1; Ras p21 protein activator; RAS p21 protein activator (GTPase activating protein) 1; RASA; RASGAP; triphosphatase-activating protein

Description (eCommerce)

RASA1 is an inhibitory regulator of the Ras-cyclic AMP pathway. It Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1Â [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P20936].

Description (Vector)

RASA1 is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.

Gene ID

5921

Gene Name (eCommerce)

RAS p21 protein activator 1

Gene Name (Vector)

RAS p21 protein activator 1

Gene Symbol

RASA1

HGNC ID

HGNC:9871

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

3144

ORF Size (bp)

3144 bp

Protein Name (eCommerce)

Ras GTPase-activating protein 1

RefSeq ID

NM_022650

RefSeq Synonyms

NM_022650, NM_002890, BC033015,

Research Areas

Apoptosis,Axon Guidance,B-cell Receptor Signaling,Cancer,Cardiovascular,EGFR Signaling,EPO Signaling,Fc Receptor Signaling,HGF Signaling,Host-Virus Interactions,IGF-1 Signaling,Immunology,Insulin Signaling,Interleukin Signaling,MAPK Signaling,Neurobiology,Neurodevelopment,PDGF Signaling,Prostate Cancer,RNA Processing,SCF-KIT Signaling,Signal Transduction,T-cell Receptor Signaling,TPO Signaling,VEGF Signaling

Research Areas (Faceted)

cancer,cardiovascular,cell_biology,developmental_biology,genetics,immunology,infectious_disease,neurobiology,signal_transduction,stem_cell_biology,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.664080

UniProt ID (eCommerce)

P20936

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.