PUMA (DBH3) Adenovirus

Name 
Ad-PUMA (DBH3)
Cat No 
1289
Availability
Immediate
Categories
Adenovirus, Over-Expression

PUMA (p53 up-regulated modulator of apoptosis) is a BH3-only protein. Each of the BH3-only group of proteins contains an amphipathic Bcl-2 homology (BH) domain. These proteins act as sensors of discrete apoptotic stimuli, antagonizing or activating the function of multidomain Bcl-2 family proteins by interacting with them. This in turn triggers a cascade of downstream events, such as the collapse of mitochondrial membrane potential, release of the apoptogenic mitochondrial proteins cytochrome c and SMAC, and activation of caspases.

PUMA plays a vital role in mediating the apoptotic responses to p53. It can be activated by p53 directly through p53-responsive elements in its promoter region. The PUMA protein interacts with Bcl-XL and promotes mitochondrial translocation and multimerization of Bax.

This adenovirus expresses human PUMA gene with a 15-aa deletion of the BH3 domain.

Reference: Yu J., et al., Mol Cell. 2001 Mar;7(3):673-82. PUMA induces the rapid apoptosis of colorectal cancer cells.

1289
Ad-PUMA (DBH3)

Ready-to-use PUMA (DBH3) Adenovirus. BBC3 PUMA (DBH3) 1289

$475.00
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Product Details

Promoter
CMV (ubiquitous)
Reporter
eGFP
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
FLJ42994; JFY1; PUMA;
Description (Vector)
BBC3 encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.
Gene ID
27113
Gene Symbol
BBC3
ORF Size (bp)
582 bp
RefSeq ID
NM_014417
RefSeq Synonyms
NM_014417, NM_001127242, NM_001127241, NM_001127240, BC136481,
Species
human

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.