Cre Recombinase Adenovirus
Cat. No: 1045
Availability:
Immediate
Name:
Ad-CMV-iCre
This Cre recombinant adenovirus expresses Cre recombinase, a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cultured cells or experimental animals.
This adenovirus expresses a codon improved Cre (iCre) under a CMV promoter.
Check out this instruction video on using our adenovirus Cre to knockout LoxP flanked gene in primary mouse embryonic fibroblasts (MEF).
$475.00
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Viral Details
- Viral Backbone
- Human Adenovirus Type5 (dE1/E3)
- Promoter
- CMV (ubiquitous)
- Storage Buffer
- DMEM, 2% BSA, 2.5% Glycerol
- Volume
- 200ul
- Titer
- 1x10^10 PFU/ml
Gene Details
- Gene Symbol
- iCre (codon optimized Cre)
Product Citations
This product is referenced in the following publications:
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- Mehlika Hazar-Rethinam, etc (2014). A novel E2F/Sphingosine kinase 1 axis regulates anthracycline response in squamous cell carcinoma. Clinical Cancer Research.
- M Song, etc (2015). Mitochondrial Fission and Fusion Factors Reciprocally Orchestrate Mitophagic Culling in Mouse Hearts and Cultured Fibroblasts. Cell Metabolism.
- Peng T, etc (2009). Jnk1/C-Fos Inhibits Cardiomyocyte Tnf-Alpha Expression Via A Negative Crosstalk With Erk And P38 Mapk In Endotoxaemia. Cardiovascular Research.
- Madhavika N. Serasinghe, etc (2015). Mitochondrial Division Is Requisite to RAS-Induced Transformation and Targeted by Oncogenic MAPK Pathway Inhibitors. Molecular Cell.
- Fulzele, K. etc (2007). Disruption Of The Insulin-Like Growth Factor Type 1 Receptor In Osteoblasts Enhances Insulin Signaling And Action. J. Biol. Chem.
- Choo MK, etc (2009). Nfatc1 Mediates Hdac-Dependent Transcriptional Repression Of Osteocalcin Expression During Osteoblast Differentiation. Bone.
- Hyejin Choi, etc (2015). Transcriptome Analysis of Individual Stromal Cell Populations Identifies Stroma-Tumor Crosstalk in Mouse Lung Cancer Model. Cell Reports.
- SV Fedeles,etc (2015). Sec63 and Xbp1 regulate IRE1a activity and polycystic disease severity. JCI.
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- LB Schook, etc (2015). A Genetic Porcine Model of Cancer. PLoS ONE.
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- Heng Wang, etc (2015). Turning terminally differentiated skeletal muscle cells into regenerative progenitors. Nature Communications.
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- Bum-Chan Park, etc (2015). The clathrin-binding and J-domains of GAK support the uncoating and chaperoning of clathrin by Hsc70 in the brain. Journal of cell Science.
- Kim DY, etc (2015). Functional regulation of FoxO1 in neural stem cell differentiation. Cell Death & Differentiation.
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- Angela Hommel (2010). The ARF-Like GTPase ARFRP1 Is Essential for Lipid Droplet Growth and Is Involved in the Regulation of Lipolysis. Molecular and Cellular Biology.
- RJ Huebner, NM Neumann, AJ Ewald (2016). Mammary epithelial tubes elongate through MAPK-dependent coordination of cell migration. Development.
- B Jung, etc (2016). Pitchfork and Gprasp2 Target Smoothened to the Primary Cilium for Hedgehog Pathway Activation. PLoS ONE.
- Ernesto Canaliskdss (2010). Connective Tissue Growth Factor Is Required for Skeletal Development and Postnatal Skeletal Homeostasis in Male Mice. Endocrinology.
- Donatella Malanga, etc (2016). AKT1E17K Is Oncogenic in Mouse Lung and Cooperates with Chemical Carcinogens in Inducing Lung Cancer. PLoS ONE.
- Kyle D, etc (2016). Serine 302 Phosphorylation of Mouse Insulin Receptor Substrate 1 (Irs1) Is Dispensable For Normal Insulin Signaling and Feedback Regulation by Hepatic S6 Kinase. JBC.
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- Tsuyoshi Shimo, etc (2016). The Role of Sonic Hedgehog Signaling in Osteoclastogenesis and Jaw Bone Destruction. PLoS ONE.
- Hua Su (2009). Reduced Expression of Integrin {alpha} v {beta} 8 Is Associated with Brain Arteriovenous Malformation Pathogenesis. American Journal of Pathology.
- Suphansa Sawamiphak (2010). Ephrin-B2 regulates VEGFR2 function in developmental and tumour angiogenesis. Nature.
- W Bouaziz, etc (2016). Interaction of HIF1a and ß-catenin inhibits matrix metalloproteinase 13 expression and prevents cartilage damage in mice. PNAS.
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- SM Brooker, AM Bond, CY Peng, JA Kessler (2016). ß1-integrin restricts astrocytic differentiation of adult hippocampal neural stem cells. GLIA.
- Elena Ambrogini (2010). FoxO-Mediated Defense against Oxidative Stress in Osteoblasts Is Indispensable for Skeletal Homeostasis in Mice. Cell Metabolism.
- Robert A. Lindquist, etc (2016). Identification of proliferative progenitors associated with prominent postnatal growth of the pons. Nature Communications.
- S Ghadakzadeh, etc (2016). ß-Catenin modulation in neurofibromatosis type 1 bone repair: therapeutic implications. The FASEB Journal.
- Li Ma, etc (2016). Integrin ß8 Deletion Enhances Vascular Dysplasia and Hemorrhage in the Brain of Adult Alk1 Heterozygous Mice. Translational Stroke Research.
- Fan Y, etc (2017). Parathyroid Hormone Directs Bone Marrow Mesenchymal Cell Fate. Cell Metabolism.
- Ma L, etc (2016). Integrin ß 8 Deletion Enhances Vascular Dysplasia and Hemorrhage in the Brain of Adult Alk1 Heterozygous Mice. Translational Stroke Research.
- Bauer M,. etc (2016). Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging. EMBO Journal.
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- Li Z, etc (2016). Glucose Transporter-4 Facilitates Insulin-stimulated Glucose Uptake in Osteoblasts. Endocrinology.
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