Adenovirus Amplification

Delivering high-yield, purified adenovirus amplification services to support your research with precision and reliability.

Why Use the Adenovirus
Amplification Service?

Large-Scale Production

To generate sufficient quantities of adenovirus for experiments requiring high viral titers.

Reliable Quality

To ensure consistency and purity, minimizing variability in experimental results.

Custom Gene Delivery

To amplify adenoviruses tailored with specific genetic constructs for precise research applications.

Efficient Workflow

To save time and resources with a streamlined amplification process managed by experts.

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Adenovirus Amplification & CsCl Purification Process

Large Scale Adenovirus
Amplification & CsCl Purification

Upon completion, you will receive a final viral stock of approximately 5×1012 to 1×1013 viral particles (~1-3×1011 PFU)*.

2-3 weeks to complete

Medium Scale Adenovirus
Amplification & Titration

Upon completion, you will receive a final viral stock of approximately 2ml with titer of 1-5×1010 PFU/IFU *.

2-3 weeks to complete

PROCESS

4-5 weeks to complete

Cloning

Transfection

Adenovirus Packaging

Amplification

CsCI Purification

PFU Titration

Custom plasmids
in 2 weeks

Viral packaging, purification, and QC in 2-4 weeks

Custom plasmids in 2 weeks

VIRAL PACKAGING, PURIFICATION, AND QC IN 2-4 WEEKS

Cloning

Transfection

Adenovirus Packaging

Amplification

Amplification

CsCI Purification

PFU Titration

How to Get Started

Large Scale Adenovirus
Amplification & CsCl Purification

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This service is ideal for those who require recombinant adenovirus for in vivo studies or just require high-titer purified viruses.

First, we infect ~4000cm2 of 293 cell monolayer through a series of amplifications from customer-provided viral stock or any adenovirus from our catalog. Then, once cytopathic effects begin to manifest, we harvest and lyse the cells to release the virus. Next, the adenovirus is purified via centrifugation using two sequential cesium chloride gradients. The final product is desalted, then titered both spectrophotometrically for viral particles and via plaque formation assay for PFU/IFU. Upon completion, you will receive a final viral stock of approximately 5×1012 to 1×1013 viral particles (~1-3×1011 PFU)*. This process takes ~2-3 weeks to complete.

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Medium Scale Adenovirus
Amplification & Titration

Continue

This service is for those who require recombinant adenovirus for in vitro studies.

The process is similar to the large scale amplification. First, we infect ~1000cm2 of HEK293 cell monolayer through a series of amplifications from customer-provided viral stock or any adenovirus from our catalog. Once cytopathic effects begin to manifest, we harvest and lyse the cells to release the virus. Then, the final product is titered for PFU/IFU. Upon completion, you will receive a final viral stock of approximately 2ml with titer of 1-5×1010 PFU/IFU *. This process takes ~2 weeks to complete. * Final viral yield may depend on the transgene in the virus.

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