HSP70 alleviates PC-12 cell oxidative damage caused by ROS through activation of Wnt/beta-catenin signaling pathway in spinal cord injury

Shi K, etc
Int J Clin Exp Patho, 2016

Spinal cord injury (SCI) is a severe and worldwide clinical problem, researches focusing on SCI for overall analysis and understanding of the mechanism will be of great importance. In the present study, we sought to explore the protective effects of HSP70 on H2O2 induced the PC-12 cell proliferation reducing and apoptosis increasing. The 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyltetrazolium bromide (MTT), flow cytometry and qRT-PCR were used to test the influence of HSP70 on cells viability, apoptosis and the ROS activity. The results showed that HSP70 promoted cell proliferation and reduced cell apoptosis, protected against PC-12 from ROS damage. Western blot results proved that overexpression of HSP70 could activate the downstream Wnt/beta (ß)-catenin signaling pathway, inhibit apoptosis factor expression. In conclusion, HSP70 promoted cell proliferation and reduced cell apoptosis, protected PC-12 cells from ROS damage via Wnt/ß-catenin signaling pathway. This paper preliminary studied the role of HSP70 in nerve cells oxidative damage, which might provide theoretical basis for the treatment of SCI.

Read more »

Int J Clin Exp Patho
doi: 9(12) :12581-12587
Qilu Hospital of Shandong University,