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mouse TSEN34 Adenovirus

Name 
Ad-m-TSEN34
Cat No 
ADV-275132
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Mouse Tsen34.

ADV-275132
Ad-m-TSEN34

Ready-to-use Adenovirus expressing Mouse Tsen34 (tRNA splicing endonuclease subunit 34). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
0610027F08Rik; Leng5
Description (Vector)
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.
Gene ID
66078
Gene Name (Vector)
tRNA splicing endonuclease subunit 34
Gene Symbol
Tsen34
ORF Size (aa)
951
ORF Size (bp)
951 bp
RefSeq ID
NM_024168
RefSeq Synonyms
NM_024168, NM_001164205, NM_001164204, BC091756, BC002205,
Species
mouse
UniGene ID
Mm.379100

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.