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human BRCC3 Over-expression AAV

Name 
AAV-h-BRCC3
Cat No 
AAV-202252
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human BRCC3.

AAV-202252
AAV-h-BRCC3

Ready-to-use AAV expressing Human BRCC3 (BRCA1/BRCA2-containing complex subunit 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRCA1-A complex subunit BRCC36; BRCC36; BRISC complex subunit BRCC36; C6.1A; CXorf53; lys-63-specific deubiquitinase BRCC36
Description (eCommerce)
BRCA1/BRCA2-containing complex subunit 36 (BRCC36) is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. BRCC36 is a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites and leads to targeting of the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, BRCC36 specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P46736].
Description (Vector)
BRCC3 encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5.
Gene ID
79184
Gene Name (eCommerce)
BRCA1/BRCA2-containing complex subunit 3
Gene Name (Vector)
BRCA1/BRCA2-containing complex subunit 3
Gene Symbol
BRCC3
HGNC ID
HGNC:24185
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
951
ORF Size (bp)
951 bp
Protein Name (eCommerce)
Lys-63-specific deubiquitinase BRCC36
RefSeq ID
NM_024332
RefSeq Synonyms
NM_024332, NM_001242640, NM_001018055, BC006540, BC002999,
Research Areas
Cancer,Cell Cycle,DNA Damage/Repair,Histone Modification,Homologous Recombination,Ubiquitination
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
BRCA1/BRCA2-containing complex subunit 36 (BRCC36) is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. BRCC36 is a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites and leads to targeting of the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, BRCC36 specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P46736].
UniGene ID
Hs.558537
UniProt ID (eCommerce)
P46736

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.