human ARMET Over-expression AAV

Name 
AAV-h-ARMET
Cat No 
AAV-201445
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human MANF.

AAV-201445
AAV-h-ARMET

Ready-to-use AAV expressing Human MANF (mesencephalic astrocyte derived neurotrophic factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
Arginine-rich protein; arginine-rich, mutated in early stage tumors; ARMET; ARP; mesencephalic astrocyte-derived neurotrophic factor; Protein ARMET
Description (eCommerce)
Mesencephalic astrocyte-derived neurotrophic factor (MANF) is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression MANF increases susceptibility to ER stress-induced death and results in cell proliferation. MANF activity is important in promoting the survival of dopaminergic neurons [taken from NCBI Entrez Gene (Gene ID: 7873)].
Description (Vector)
MANF is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region, including a specific mutation that changes the previously numbered codon 50 from ATG to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related.
Gene ID
7873
Gene Name (eCommerce)
mesencephalic astrocyte derived neurotrophic factor
Gene Name (Vector)
mesencephalic astrocyte derived neurotrophic factor
Gene Symbol
MANF
HGNC ID
HGNC:15461
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
558
Protein Name (eCommerce)
Mesencephalic astrocyte-derived neurotrophic factor
RefSeq ID
NM_006010
RefSeq Synonyms
NM_006010, BC113590, BC113588, BC007282,
Research Areas
Protein Folding,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology
Species
human
Target Sentence
Mesencephalic astrocyte-derived neurotrophic factor (MANF) is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression MANF increases susceptibility to ER stress-induced death and results in cell proliferation. MANF activity is important in promoting the survival of dopaminergic neurons [taken from NCBI Entrez Gene (Gene ID: 7873)].
UniGene ID
Hs.436446
UniProt ID (eCommerce)
P55145

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.