human NANS Adenovirus

Name
Ad-h-NANS

Cat No
ADV-216303

Availability
4-5 weeks

This is an Adenovirus expressing Human NANS.

ADV-216303

Ad-h-NANS

Ready-to-use Adenovirus expressing Human NANS (N-acetylneuraminate synthase). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Vector Designer

Product Details

Promoter

CMV

Reporter

none, optional GFP, CFP, YFP, RFP or mCherry

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

HEL-S-100; SAS; SEMDCG; SEMDG

Description (Vector)

NANS encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. NANS is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.

Gene ID

54187

Gene Name (Vector)

N-acetylneuraminate synthase

Gene Symbol

NANS

HGNC ID

HGNC:19237

ORF Size (aa)

1080

ORF Size (bp)

1080 bp

RefSeq ID

NM_018946

RefSeq Synonyms

NM_018946, BC019315, BC000008,

Species

human

UniGene ID

Hs.665191

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.