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human MED12 Adenovirus

Name 
Ad-h-MED12
Cat No 
ADV-215234
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human MED12.

ADV-215234
Ad-h-MED12

Ready-to-use Adenovirus expressing Human MED12 (mediator complex subunit 12). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; CAGH45; FGS1; HOPA; human opposite paired; MED12S; Mediator complex subunit 12; mediator of RNA polymerase II transcription subunit 12; mediator of RNA polymerase II transcription, subunit 12 homolog; OHDOX; OKS; OPA1; OPA-containing protein; putative mediator subunit 12; thyroid hormone receptor-associated protein complex 230 kDa component; thyroid hormone receptor-associated protein, 230 kDa subunit; TNRC11; TRAP230; trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); trinucleotide repeat-containing gene 11 protein
Description (eCommerce)
MED12 is a component of the multisubunit mediator complex that functions as an interface between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. In this context, MED12 interaction with beta-catenin has been shown to be required for Wnt-signaling dependent transcription.
Description (Vector)
The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.
Gene ID
9968
Gene Name (eCommerce)
mediator complex subunit 12
Gene Name (Vector)
mediator complex subunit 12
Gene Symbol
MED12
HGNC ID
HGNC:11957
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
6531
ORF Size (bp)
6531 bp
Protein Name (eCommerce)
Mediator of RNA polymerase II transcription subunit 12
RefSeq ID
NM_005120
RefSeq Synonyms
NM_005120, BC156978, BC156136,
Research Areas
Gene Expression,Hedgehog Signaling,Metabolism/Metabolic Process,Neurobiology,Nuclear Receptor Coactivators,Signal Transduction,Transcription Factor/Regulator,Wnt Signaling
Research Areas (Faceted)
cell_biology,metabolism,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
MED12 is a component of the multisubunit mediator complex that functions as an interface between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. In this context, MED12 interaction with beta-catenin has been shown to be required for Wnt-signaling dependent transcription.
UniGene ID
Hs.409226
UniProt ID (eCommerce)
Q93074

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.