human MANEA Adenovirus

Name 
Ad-h-MANEA
Cat No 
ADV-214888
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human MANEA.

ADV-214888
Ad-h-MANEA

Ready-to-use Adenovirus expressing Human MANEA (mannosidase endo-alpha). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ENDO; hEndo
Description (Vector)
N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 (PubMed 15677381)).(supplied by OMIM, Sep 2008)
Gene ID
79694
Gene Name (Vector)
mannosidase endo-alpha
Gene Symbol
MANEA
HGNC ID
HGNC:21072
ORF Size (aa)
1386
ORF Size (bp)
1386 bp
RefSeq ID
NM_024641
RefSeq Synonyms
NM_024641, BC150199, BC146689, BC146671, BC137016, BC137014,
Species
human
UniGene ID
Hs.533323

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.