human BID shRNA Adenovirus

Name 
Ad-h-BID-shRNA
Cat No 
1812
Availability
Immediate
Categories
Adenovirus, shRNA-Silencing

This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human BID gene. The shRNA expression is driven by an U6 promoter.

The knockdown of this human gene was validated by western blot in A549 cells.

1812
Ad-h-BID-shRNA

Ready-to-use human BID shRNA Adenovirus. human BID shRNA adenovirus gene knockdown1812

$995.00
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Product Details

Promoter
U6 ()
Reporter
eGFP
shRNA Knockdown (%)
85
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
apoptic death agonist; BH3 interacting domain death agonist Si6 isoform; BH3-interacting domain death agonist; BID; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; FP497; Human BID coding sequence; p22 BID
Description (eCommerce)
BID (BH3-interacting domain death agonist) can mediate both apoptotic and pro-survival signals in response to DNA damage. BID is phosphorylated by ATM in response to DNA damage and may play a role in the cell’s decision of whether to live or die based on the extent of damage.
Description (Vector)
BID encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
Gene ID
637
Gene Name (eCommerce)
BH3 interacting domain death agonist
Gene Name (Vector)
BH3 interacting domain death agonist
Gene Symbol
BID
HGNC ID
HGNC:1050
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
588
ORF Size (bp)
588 bp
Protein Name (eCommerce)
BH3-interacting domain death agonist
RefSeq ID
NM_001196
RefSeq Synonyms
NM_197967, NM_197966, NM_001244572, NM_001244570, NM_001244569, NM_001244567, NM_001196, BC036364, BC033634, BC022072, BC009197,
Research Areas
Alzheimer's Disease,Amyotrophic Lateral Sclerosis (ALS),Apoptosis,Brain Development,Breast Cancer,Cancer,Cardiology,Cardiovascular,CD95/FAS Signaling,Cell Cycle,DNA Damage/Repair,Host-Virus Interactions,Immunology,Mitochondrion,Neurobiology,Neurodegeneration,Neurodevelopment,p53 Signaling,Signal Transduction,TNF Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,immunology,infectious_disease,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
BID (BH3-interacting domain death agonist) can mediate both apoptotic and pro-survival signals in response to DNA damage. BID is phosphorylated by ATM in response to DNA damage and may play a role in the cell’s decision of whether to live or die based on the extent of damage.
UniGene ID
Hs.591054
UniProt ID (eCommerce)
P55957

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.