Cre Recombinase Adenovirus
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.
This adenovirus expresses both Cre recombinase and GFP as marker. Cre and GFP are driven by 2 separate promoters.
Check out this instruction video on using our adenovirus Cre to knockout LoxP flanked gene in primary mouse embryonic fibroblasts (MEF).
1700
Ad-Cre-GFP
Ready-to-use Cre Recombinase Adenovirus. Ad-Cre-GFP Cre GFP adenovirus 1700
Related Citations
- CpG Islands Shape the Epigenome Landscape. C Papin, etc, (2020), Journal of Molecular Biology
- INTEGRIN-LINKED KINASE REGULATES MELANOSOME TRAFFICKING AND MELANIN TRANFER IN MELANOCYTES.. M Crawford, etc, (2020), Molecular Biology of the Cell
- Direct Comparison of Mononucleated and Binucleated Cardiomyocytes Reveals Molecular Mechanisms Underlying Distinct Proliferative Competencies. R Windmueller, etc, (2020), Cell Reports
- Synthetic Lethality between DNA Polymerase Epsilon and RTEL1 in Metazoan DNA Replication. R Bellelli, etc, (2020), Cell Reports
- Subventricular zone adult mouse neural stem cells and human glioblastoma stem cells require insulin receptor for self-renewal. S Chidambaram, etc, (2020), bioRxiv
- Metallothioneins regulate ATP7A trafficking and control cell viability during copper deficiency and excess. N Gudekar, etc, (2020), Scientific Reports
- IKKa Kinase Regulates the DNA Damage Response and Drives Chemo-resistance in Cancer. C Colomer, etc, (2019), Molecular Cell,
- CDK phosphorylation of TRF2 controls t-loop dynamics during the cell cycle. G Sarek, etc, (2019), Nature
- Single-cell transcriptome analysis identifies distinct cell types and niche signaling in a primary gastric organoid model. J Chen, etc, (2019), Scientific Reports
- Regulation of the bone vascular network is sexually dimorphic. A Goring, etc, (2019), Journal of Bone and Mineral Research
- Trimethylamine N-Oxide Binds and Activates PERK to Promote Metabolic Dysfunction. S Chen, etc, (2019), Cell Metabolism
- The BRG1/SOX9 axis is critical for acinar cell–derived pancreatic tumorigenesis. M Tsuda, etc, (2018), JCI
- Ghrelin Causes a Decline in GABA Release by Reducing Fatty Acid Oxidation in Cortex. JF Mir, etc, (2018), Molecular Neurobiology
- Deubiquitinase function of A20 maintains and repairs endothelial barrier after lung vascular injury. D Soni, etc, (2018), Cell Death Discovery
- Mammalian pigmentation is regulated by a distinct cAMP-dependent mechanism that controls melanosome pH. D Zhou, etc, (2018), Sci. Signal
- Cyclin B1 stability is increased by interaction with BRCA1, and its overexpression suppresses the progression of BRCA1-associated mammary tumors. EK Choi, etc, (2018), Experimental & Molecular Medicinevolume
- Article | OPEN | Published: 21 August 2018 Lgl1 controls NG2 endocytic pathway to regulate oligodendrocyte differentiation and asymmetric cell division and gliomagenesis. Daynac M, etc, (2018), Nature Communications
- GATA4 represses RANKL in osteoblasts via multiple long-range enhancers to regulate osteoclast differentiation. AB Khalid, etc, (2018), Bone
- The Factor Inhibiting HIF Asparaginyl Hydroxylase Regulates Oxidative Metabolism and Accelerates Metabolic Adaptation to Hypoxia. J Sim, etc, (2018), Cell Metabolism
- PDGFRa+ pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo. G Greicius, etc, (2018), PNAS
- Enhancer of zeste homolog 2 (Ezh2) controls bone formation and cell cycle progression during osteogenesis in mice. Dudakovic A, etc, (2018), JBC
- Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe. P Margalef, etc, (2017), Cell
- PPARd Promotes Running Endurance by Preserving Glucose. Fan W, etc, (2017), Cell Metabolism
- Disruption of the Cx43/miR21 pathway leads to osteocyte apoptosis and increased osteoclastogenesis with aging. Davis H, etc, (2017), Aging Cell
- Connexin43 and Runx2 Interact to Affect Cortical Bone Geometry, Skeletal Development, and Osteoblast and Osteoclast Function. http://onlinelibrary.wiley.com/doi/10.1002/jbmr.3152/full, (2017), Journal of Bone and Mineral Research
- Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control. Glytsou C, etc, (2016), Cell Reports
- N-sulfation of heparan sulfate is critical for syndecan-4 mediated podocyte cell-matrix interactions. T Sugar, etc, (2016), American Journal of Physiology
- Cardiolipin or MTCH2 can serve as tBID receptors during apoptosis. E Raemy, etc, (2016), Cell Death & Differentiation
- Autocrine effect of vascular endothelial growth factor-A is essential for mitochondrial function in brown adipocytes. Kiana Mahdaviani, etc, (2016), Metabolism
- Formation of renal cysts and tumors in Vhl/Trp53-deficient mice requires HIF-1a and HIF-2a. Désirée Schönenberger, etc, (2016), Cancer Research
- An Essential Role for Cdc42 in the Functioning of the Adult Mammary Gland. Joseph E. Druso, etc, (2016), JBC
- EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance. Depner C, etc, (2016), Nature Communications
- Establishment of Immortalized BMP2/4 Double Knock-Out Osteoblastic Cells is Essential for Study of Osteoblast Growth, Differentiation and Osteogenesis. Wu LA, etc, (2015), Journal of Cellular Physiology
- TRF2 Recruits RTEL1 to Telomeres in S Phase to Promote T-Loop Unwinding. Grzegorz Sarek, etc, (2015), Molecular Cell
- A chemogenomic screening identifies CK2 as a target for pro-senescence therapy in PTEN-deficient tumours. Madhuri Kalathur, etc, (2015), Nature Communications
- O-GlcNAcylation Negatively Regulates Cardiomyogenic Fate in Adult Mouse Cardiac Mesenchymal Stromal Cells. A Zafir, etc, (2015), PLoS ONE
- Transcriptional modulator ZBED6 affects cell cycle and growth of human colorectal cancer cells. MA A, etc, (2015), PNAS
- MiR-371-5p Facilitates Pancreatic Cancer Cell Proliferation and Decreases Patient Survival. De He, etc, (2014), PLOS One
- ATRX promotes gene expression by facilitating transcriptional elongation through guanine-rich coding regions. MA Levy, etc, (2014), Human Molecular Genetics
- Fanconi Anemia Gene Editing by the CRISPR/Cas9 System. MJ Osborn, etc, (2014), Human Gene Therapy
- Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR. AT Henze, etc, (2014), Nature Communications
- Stimulation of the soluble guanylate cyclase (sGC) inhibits fibrosis by blocking non-canonical TGFß signalling. Christian Beyer, etc, (2014), Ann Rheumatic Diseases
- Contrasting roles of E2F2 and E2F3 in endothelial cell growth and ischemic angiogenesis. Zhou J. etc, (2013), Journal of Molecular and Cellular Cardiology
- DNA-Damage-Induced Nuclear Export of Precursor MicroRNAs Is Regulated by the ATM-AKT Pathway. Guohui Wan, etc, (2013), Cell Reports
- Histone Lysine-specific Demethylase 1 (LSD1) Protein Is Involved in Sal-like Protein 4 (SALL4)-mediated Transcriptional Repression in Hematopoietic Stem Cells. Li Liu, (2013), The Journal of Biological Chemistry
- Mitochondrial Cristae Shape Determines Respiratory Chain Supercomplexes Assembly and Respiratory Efficiency. Sara Cogliati, (2013), Cell
- Runx3 Inactivation Is a Crucial Early Event in the Development of Lung Adenocarcinoma. You-Soub Lee, (2013), Cancer Cell
- Histone Demethylase LSD1 is Involved in SALL4 Mediated Transcriptional Repression in Hematopoietic Stem Cells. Li Liu, etc, (2013), The Journal of Biological Chemistry
- Protein O-GlcNAcylation is a Novel Cytoprotective Signal in Cardiac Stem Cells. Ayesha Zafir, etc, (2013), Stem Cells
- An In Vivo RNAi Screening Approach to Identify Host Determinants of Virus Replication. Andrew Varble, (2013), Cell Host & Microbe
- Combined mutation of Vhl and Trp53 causes renal cysts and tumours in mice. Albers, J. etc, (2013), EMBO Molecular Medicine
- Disruption of clathrin-mediated trafficking causes centrosome overduplication and senescence. Olszewski MB, etc, (2013), Traffic
- TALEN-based Gene Correction for Epidermolysis Bullosa. Osborn, MJ. etc, (2013), Molecular Therapy
- Repositioning Forelimb Superficialis Muscles: Tendon Attachment and Muscle Activity Enable Active Relocation of Functional Myofibers. Alice H. Huang, (2013), Developmental Cell
- Endogenous Origins of HIV-1 G-to-A Hypermutation and Restriction in the Nonpermissive T Cell Line CEM2n. Refsland, EW, etc, (2012), PLoS Pathogens
- LKB1 Destabilizes Microtubules in Myoblasts and Contributes to Myoblast Differentiation. Mian, I. etc, (2012), PLoS ONE
- Deletion of CaMKK2 from the Liver Lowers Blood Glucose and Improves Whole-Body Glucose Tolerance in the Mouse. Kristin A. Anderson, etc, (2012), Molecular Endocrinology
- Lipasin, a novel nutritionally-regulated liver-enriched factor that regulates serum triglyceride levels. Zhang R, etc, (2012), Biochem Biophys Res Commun
- Molecular mimicry rather than identity breaks T-cell tolerance in the CYP2D6 mouse model for human autoimmune hepatitis. Janine Ehser, etc, (2012), Journal of Autoimmunity
- DIRECT AND INDIRECT ROLES FOR ¿-CATENIN IN FACULTATIVE BASAL PROGENITOR CELL DIFFERENTIATION. Smith, MK. etc, (2012), American Journal of Physiology
- Precise Regulation of Porcupine Activity is Required for Physiological Wnt Signaling. Proffitt KD, etc, (2012), J Biol Chem
- Transcriptionally Active Syncytial Aggregates in the Maternal Circulation May Contribute to Circulating Soluble Fms-Like Tyrosine Kinase 1 in Preeclampsia. Augustine Rajakumar, etc, (2012), Hypertension
- Notch Activation by the Metalloproteinase ADAM17 Regulates Myeloproliferation and Atopic Barrier Immunity by Suppressing Epithelial Cytokine Synthesis. Aditya Murthy, etc, (2012), Immunity
- The Role of Dlc1 Isoform 2 in K-Ras2-G12D Induced Thymic Cancer. Sabbir, MG, etc, (2012), PLoS ONE
- Protein tyrosine phosphatase SHP2 regulates TGF-ß1 production in airway epithelia and asthmatic airway remodeling in mice. X.-J. Qin, etc, (2012), Allergy
- Protein Kinase C Iota Regulates Pancreatic Acinar-to-Ductal Metaplasia. Scotti ML, etc, (2012), PLoS ONE
- Identification of mRNAs that are spliced but not exported to the cytoplasm in the absence of THOC5 in mouse embryo fibroblasts. Guria, A. etc, (2011), RNA
- In Situ Genetic Correction of the Sickle Cell Anemia Mutation in Human Induced Pluripotent Stem Cells Using Engineered Zinc Finger Nucleases. Vittorio Sebastiano, etc, (2011), Stem Cells
- Ecrg4 expression and its product augurin in the choroid plexus: impact on fetal brain development, cerebrospinal fluid homeostasis and neuroprogenitor cell response to CNS injury. Ana Maria Gonzalez, etc, (2011), CNS
- Inactivation of ARF-BP1 induces p53 activation and the diabetic phenotypes in mice. Kon, N. etc, (2011), J Biol Chem
- TM4SF1: a tetraspanin-like protein necessary for nanopodia formation and endothelial cell migration. Zukauskas A, etc, (2011), Angiogenesis
- Fibronectin and {beta}-Catenin Act in a Regulatory Loop in Dermal Fibroblasts to Modulate Cutaneous Healing. Bielefeld KA, etc, (2011), J Biol Chem
- Phenotype of single hepatocytes expressing an activated version of ß-catenin in liver of transgenic mice. Sandra Schreiber, etc, (2011), Journal of Molecular Histology
- Role of HIF-1a in maternal hyperglycemia-induced embryonic vasculopathy. Yang P, etc, (2011), Am J Obstet Gynecol
- Dysregulation of hypoxia pathways in fumarate hydratase-deficient cells is independent of defective mitochondrial metabolism. O'Flaherty L, etc, (2010), Human Molecular Genetics
- Downregulation of the coxsackie and adenovirus receptor in cancer cells by hypoxia depends on HIF-1a. Küste K, etc, (2010), Cancer Gene Therapy
- THOC5/FMIP, an mRNA export TREX complex protein, is essential for hematopoietic primitive cell survival in vivo. Mancin A, etc, (2010), BMC Biology
- Serum-and glucocorticoid-inducible kinase 1 (SGK1) regulates adipocyte differentiation via forkhead box O1. Pietro ND, etc, (2010), Molecular Endocrinology
- Phosphorylation of the NFAR proteins by the dsRNA-dependent protein kinase PKR constitutes a novel mechanism of translational regulation and cellular defense. Harashima A, etc, (2010), Genes & Development
- Podocytes require the engagement of cell surface heparan sulfate proteoglycans for adhesion to extracellular matrices. Shoujun Chen, etc, (2010), Kidney International
- Serum- And Glucocorticoid-Inducible Kinase 1 (Sgk1) Regulates Adipocyte Differentiation Via Forkhead Box O1. Pietro, N. etc, (2010), Molecular Endocrinology
- Transcription Coactivator Pbp/Med1 Deficient Hepatocytes Are Not Susceptible To Diethylnitrosamine-Induced Hepatocarcinogenesis In The Mouse. Matsumoto K. etc, (2010), Carcinogenesis
- Reduced Expression of Integrin {alpha} v {beta} 8 Is Associated with Brain Arteriovenous Malformation Pathogenesis. Hua Su, (2009), American Journal of Pathology
- Adeno-Associated Virus and Adenovirus Coinfection Induces a Cellular DNA Damage and Repair Response via Redundant Phosphatidylinositol 3-Like Kinase Pathways. Roy F. Collaco, etc, (2009), Virology
Recently Viewed Products
Ad-CMV-iCre
Cat No: 1045
Ad-CMV-Caspase 9 (DN)
Cat No: 1044
Ad-CMV-Rb
Cat No: 1043
Ad-CMV-p27
Cat No: 1042
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.