mouse HS3ST5 Over-expression AAV

Name 
AAV-m-HS3ST5
Cat No 
AAV-261675
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Mouse Hs3st5.

AAV-261675
AAV-m-HS3ST5

Ready-to-use AAV expressing Mouse Hs3st5 (heparan sulfate (glucosamine) 3-O-sulfotransferase 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
D930005L05Rik; Gm1151; Hs3ost5
Description (Vector)
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Also generates GlcUA-GlcNS or IdoUA-GlcNS and IdoUA2S-GlcNH2. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry.
Gene ID
319415
Gene Name (Vector)
heparan sulfate (glucosamine) 3-O-sulfotransferase 5
Gene Symbol
Hs3st5
ORF Size (aa)
1041
RefSeq ID
NM_001081208
RefSeq Synonyms
NM_001253356, NM_001253355, NM_001081208, BC158004, BC150973, BC147493, BC147490,
Species
mouse
UniGene ID
Mm.332442

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.