human EGLN1 Over-expression AAV

Name 
AAV-h-EGLN1
Cat No 
AAV-233019
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human EGLN1.

AAV-233019
AAV-h-EGLN1

Ready-to-use AAV expressing Human EGLN1 (egl-9 family hypoxia inducible factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
C1orf12; ECYT3; egl nine homolog 1; egl nine-like protein 1; HALAH; HIFPH2; HIF-PH2; HIF-prolyl hydroxylase 2; HPH2; HPH-2; hypoxia-inducible factor prolyl hydroxylase 2; PHD2; prolyl hydroxylase domain-containing protein 2; SM20; SM-20; zinc finger MYND domain-containing protein 6; ZMYND6
Description (eCommerce)
PHD2 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity.
Description (Vector)
EGLN1 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
Gene ID
54583
Gene Name (eCommerce)
egl-9 family hypoxia inducible factor 1
Gene Name (Vector)
egl-9 family hypoxia inducible factor 1
Gene Symbol
EGLN1
HGNC ID
HGNC:1232
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
387
Protein Name (eCommerce)
Egl nine homolog 1
RefSeq ID
NM_022051
RefSeq Synonyms
NM_022051, BC005369,
Research Areas
Angiogenesis,Cancer,Cardiac Development,Cardiology,Cardiovascular,HIF-1 Signaling,Hypoxia,Kidney Cancer,Signal Transduction,Transcription Factor/Regulator,Ubiquitination,Zinc-finger
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,signal_transduction,transcription_translation
Species
human
Target Sentence
PHD2 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity.
UniProt ID (eCommerce)
Q9GZT9

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.