human TRIM21 Over-expression AAV

Name 
AAV-h-TRIM21
Cat No 
AAV-226410
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human TRIM21.

AAV-226410
AAV-h-TRIM21

Ready-to-use AAV expressing Human TRIM21 (tripartite motif containing 21). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; RING-type E3 ubiquitin transferase TRIM21; RNF81; ro(SS-A); Ro/SSA; RO52; Sicca syndrome antigen A; sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SSA; SS-A; SSA1; tripartite motif-containing protein 21
Description (eCommerce)
Sjoegren syndrome type A antigen (SSA) is a component of a ribonucleoprotein particle composed of a single polypeptide and one of four small RNA molecules. It is present in all mammalian cells studied but has no known function. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P19474].
Description (Vector)
TRIM21 encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
Gene ID
6737
Gene Name (eCommerce)
tripartite motif containing 21
Gene Name (Vector)
tripartite motif containing 21
Gene Symbol
TRIM21
HGNC ID
HGNC:11312
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1428
ORF Size (bp)
1428 bp
Protein Name (eCommerce)
E3 ubiquitin-protein ligase TRIM21
RefSeq ID
NM_003141
RefSeq Synonyms
NM_003141, BC010861,
Research Areas
Antigen Processing and Presentation,Cell Cycle,Immunology,Innate Immunity,RNA Binding,RNA Processing,Zinc-finger
Research Areas (Faceted)
cell_cycle,immunology,genetics,transcription_translation,cell_biology
Species
human
Target Sentence
Sjoegren syndrome type A antigen (SSA) is a component of a ribonucleoprotein particle composed of a single polypeptide and one of four small RNA molecules. It is present in all mammalian cells studied but has no known function. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P19474].
UniGene ID
Hs.532357
UniProt ID (eCommerce)
P19474

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.