human PLOD1 Over-expression AAV

Name
AAV-h-PLOD1

Cat No
AAV-219218

Availability
4-5 weeks

This is an AAV expressing Human PLOD1.

AAV-219218

AAV-h-PLOD1

Ready-to-use AAV expressing Human PLOD1 (procollagen-lysine,2-oxoglutarate 5-dioxygenase 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

CMV (default), or choose from 30 different ubiquitous or cell specific promoter

Reporter

none, optional GFP, CFP, YFP, RFP or mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

EDS6; LH; LH1; LLH; PLOD

Description (Vector)

Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.

Gene ID

5351

Gene Name (Vector)

procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Gene Symbol

PLOD1

HGNC ID

HGNC:9081

ORF Size (aa)

2184

ORF Size (bp)

2184 bp

RefSeq ID

NM_000302

RefSeq Synonyms

NM_001316320, NM_000302, BC016657,

Species

human

UniGene ID

Hs.75093

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.