AAV with EF1a promoter driven hChR2(T159C)-TdTomato

Name 
AAV-EF1a-hChR2(T159C)-TdTomato
Cat No 
VB4725
Availability
3 weeks
Categories
Optogenetics, AAV, Over-Expression

This AAV expresses hChR2(T159C)-TdTomato driven by an ubiquitous EF1a promoter.

The 1.2 Kb EF1α promoter is a commonly used, constitutive promoter derived from human elongation factor-1 alpha (EF1α). It is often used in conditions where the other constitutive promoters, such as CMV, have diminished activity or have been silenced.

hChR2 is a humanized version of ChR2 for mammalian expression. The wild-type, as well as a few mutations, provide the fastest excitation of the channelrhodopsins offered, and are widely used in optogenetics techniques in neuroscience. hChR2(T159C) shows slightly higher photocurrents than the hChR2(H134R), another commonly used optogenetics tool.

VB4725
AAV-EF1a-hChR2(T159C)-TdTomato

Ready-to-use AAV expressing hChR2(T159C)-TdTomato driven by an ubiquitous EF1a promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
EF1a (ubiquitous)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.