AAV with CMV-IVS promoter driven C1V1(E122T/E162T)-TS-TdTomato
This AAV expresses C1V1(E122T/E162T)-TS-TdTomato driven by an ubiquitous CMV-IVS promoter.
The CMV-IVS promoter is about 1.1 kb in length and consists of the commonly used CMV promoter/enhancer and the intron (IVS) from beta-globin . The presence of intron elements was shown to give a higher level of transgene expression than that of the original CMV promoter/enhancer.
C1V1 is a chimeric channelrhodopsin made to boost membrane expression higher than VChR1 but with a different light spectrum. It is composed of ChR1 and VChR1 fragments, and implements fast, potent optical excitation at red-shifted wavelengths. C1V1(E122T/162T) is highly efficient in neurons, with a maximum absorption at 530nM with a narrow action spectra. Off-kinetics is 35ms.
The trafficking signal (KSRITSEGEYIPLDQIDINV) is also from the inward rectifier potassium channel Kir2.1; it serves to dramatically reduce intracellular accumulation and improve membrane targeting, leading to a profound increase of photocurrents. This is the basis for many third-generation optogenetics tools.
Ready-to-use AAV expressing C1V1(E122T/E162T)-TS-TdTomato driven by an ubiquitous CMV-IVS promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.