AAV with CMV promoter driven hChR2(E123T)-TdTomato

Name
AAV-cDNA6-hChR2(E123T)-TdTomato

Cat No
VB3182

Availability
2-3 weeks

Categories
Optogenetics, AAV, Over-Expression

This AAV expresses hChR2(E123T)-TdTomato driven by an ubiquitous CMV promoter.

This roughly 0.6 Kb promoter is the commonly used human cytomegalovirus (CMV) immediate-early promoter/enhancer (CMV/IE).

hChR2 is a humanized version of ChR2 for mammalian expression. Wild-type ChR2, as well as a few of its mutations, provides the fastest excitation of the channelrhodopsins offered, and is widely used in in optogenetics techniques in neuroscience. hChR2(E123T) mutant accelerates the open state decay but reduces photocurrent amplitudes. Its maximal spectrum is near 495nM.

VB3182

AAV-cDNA6-hChR2(E123T)-TdTomato

Ready-to-use AAV expressing hChR2(E123T)-TdTomato driven by an ubiquitous CMV promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

CMV (ubiquitous)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume

100 µl, 250 µl, 500 µl, and 1ml

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.