AAV with ApoE/hAAT1 promoter driven CreERT2

Name 
AAV-ApoE/hAAT1-CreERT2-WPRE
Cat No 
VB2000
Availability
2-3 weeks
Categories
AAV, Cre Recombinases, Over-Expression

This AAV expresses CreERT2 driven by a liver ApoE/hAAT1 promoter.

The ApoE/AAT1 chimeric promoter consists of the core promoter from human alpha-1-antitrypsin gene (also known as hAAT1, SERPINA1, A1A, A1AT, AAT, or alpha1AT) and the enhancer/hepatic locus control region (HCR) from the ApoE gene. The ApoE-hAAT1 chimeric promoter is about 0.7Kb and is used for driving gene expression in hepatocytes

A mutated form of estrogen ligand-binding domain (ERT2) that binds to synthetic antagonists (such as tamoxifen or its derivative 4-hydroxy-tamoxifen) but not to circulating estrogen, was fused to the Cre recombinase (Cre) to create CreERT2 (also known as “inducible Cre”). When tamoxifen binds to CreERT2, it induces a conformational change of CreERT2, leading to its nuclear translocation, followed by Cre/Lox-mediated recombination.

VB2000
AAV-ApoE/hAAT1-CreERT2-WPRE

Ready-to-use AAV expressing CreERT2 driven by a liver ApoE/hAAT1 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
ApoE/hAAT1 (liver)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume
100 µl, 250 µl, 500 µl, and 1ml

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.