rat QTRT1 shRNA silencing Adenovirus

Name 
Ad-r-QTRT1-shRNA
Cat No 
shADV-294594
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Qtrt1.

shADV-294594
Ad-r-QTRT1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Qtrt1 (queuine tRNA-ribosyltransferase catalytic subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
61
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Tgt; Tgut
Description (Vector)
Interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity).
Gene ID
64016
Gene Name (Vector)
queuine tRNA-ribosyltransferase catalytic subunit 1
Gene Symbol
Qtrt1
ORF Size (aa)
1212
ORF Size (bp)
1212 bp
RefSeq ID
NM_022250
RefSeq Synonyms
NM_022250, BC097321,
Species
rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.