rat NEIL1 shRNA silencing Adenovirus

Name 
Ad-r-NEIL1-shRNA
Cat No 
shADV-294318
Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Neil1.

shADV-294318
Ad-r-NEIL1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Neil1 (nei-like DNA glycosylase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches.
Gene ID
367090
Gene Name (Vector)
nei-like DNA glycosylase 1
Gene Symbol
Neil1
ORF Size (aa)
1164
ORF Size (bp)
1164 bp
RefSeq ID
NM_001025754
RefSeq Synonyms
NM_001025754, BC099125,
Species
rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.