rat ATG4B shRNA silencing Adenovirus

Name 
Ad-r-ATG4B-shRNA
Cat No 
shADV-294229
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Atg4b.

shADV-294229
Ad-r-ATG4B-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Atg4b (autophagy related 4B, cysteine peptidase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
71
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Apg4b
Description (Vector)
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Gene ID
316640
Gene Name (Vector)
autophagy related 4B, cysteine peptidase
Gene Symbol
Atg4b
ORF Size (aa)
675
ORF Size (bp)
675 bp
RefSeq ID
NM_001025711
RefSeq Synonyms
NM_001025711, BC098833,
Species
rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.