rat CAST shRNA silencing Adenovirus

Name 
Ad-r-CAST-shRNA
Cat No 
shADV-293405
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Cast.

shADV-293405
Ad-r-CAST-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Cast (calpastatin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Gene ID
25403
Gene Name (Vector)
calpastatin
Gene Symbol
Cast
ORF Size (aa)
2028
ORF Size (bp)
2028 bp
RefSeq ID
NM_053295
RefSeq Synonyms
NM_053295, NM_001033716, NM_001033715, BC091239,
Species
rat

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.