rat RGD1309888 shRNA silencing Adenovirus

Name 
Ad-r-RGD1309888-shRNA
Cat No 
shADV-292440
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Smg9.

shADV-292440
Ad-r-RGD1309888-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Smg9 (SMG9 nonsense mediated mRNA decay factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
91
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
RGD1309888
Description (Vector)
Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG8 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required for the efficient association between SMG1 and SMG8.
Gene ID
365215
Gene Name (Vector)
SMG9 nonsense mediated mRNA decay factor
Gene Symbol
Smg9
ORF Size (aa)
1563
ORF Size (bp)
1563 bp
RefSeq ID
NM_001014243
RefSeq Synonyms
NM_001014243, BC087051,
Species
rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.