rat RGD1309216 shRNA silencing Adenovirus

Name 
Ad-r-RGD1309216-shRNA
Cat No 
shADV-292387
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Sdhaf2.

shADV-292387
Ad-r-RGD1309216-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Sdhaf2 (succinate dehydrogenase complex assembly factor 2). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
RGD1309216
Description (Vector)
Required for insertion of FAD cofactor into SDHA, the catalytic subunit of succinate dehydrogenase (SDH). SDH is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). In is unclear whether it participates in the chemistry of FAD attachment (enzymatic function) or acts as a chaperone that maintains SDHA in a conformation that is susceptible to autocatalytic FAD attachment.
Gene ID
361726
Gene Name (Vector)
succinate dehydrogenase complex assembly factor 2
Gene Symbol
Sdhaf2
ORF Size (aa)
495
RefSeq ID
NM_001008371
RefSeq Synonyms
NM_001008371, BC086542,
Species
rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.