rat NDUFS1 shRNA silencing Adenovirus

Name
Ad-r-NDUFS1-shRNA

Cat No
shADV-292043

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Ndufs1.

shADV-292043

Ad-r-NDUFS1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Ndufs1 (NADH dehydrogenase (ubiquinone) Fe-S protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.

Gene ID

301458

Gene Name (Vector)

NADH dehydrogenase (ubiquinone) Fe-S protein 1

Gene Symbol

Ndufs1

ORF Size (aa)

2184

ORF Size (bp)

2184 bp

RefSeq ID

NM_001005550

RefSeq Synonyms

NM_001005550, BC081892,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.