rat AATF shRNA silencing Adenovirus

Name 
Ad-r-AATF-shRNA
Cat No 
shADV-291949
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Aatf.

shADV-291949
Ad-r-AATF-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Aatf (apoptosis antagonizing transcription factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
84
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Ded
Description (Vector)
AATF may function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Gene ID
114512
Gene Name (Vector)
apoptosis antagonizing transcription factor
Gene Symbol
Aatf
ORF Size (aa)
1572
ORF Size (bp)
1572 bp
RefSeq ID
NM_053720
RefSeq Synonyms
NM_053720, BC078769,
Species
rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.