rat NHEJ1 shRNA silencing Adenovirus

Name 
Ad-r-NHEJ1-shRNA
Cat No 
shADV-291149
Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Nhej1.

shADV-291149
Ad-r-NHEJ1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Nhej1 (nonhomologous end-joining factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)
DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.
Gene ID
363251
Gene Name (Vector)
nonhomologous end-joining factor 1
Gene Symbol
Nhej1
ORF Size (aa)
915
ORF Size (bp)
915 bp
RefSeq ID
NM_001014217
RefSeq Synonyms
NM_001014217, BC079033,
Species
rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.