mouse USP5 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Usp5.
shADV-275751
Ad-m-USP5-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Usp5 (ubiquitin specific peptidase 5 (isopeptidase T)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AA407472; ISOT; ISOT-1; Ucht
Description (Vector)
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Gene ID
22225
Gene Name (Vector)
ubiquitin specific peptidase 5 (isopeptidase T)
Gene Symbol
Usp5
ORF Size (aa)
2577
ORF Size (bp)
2577 bp
RefSeq ID
NM_013700
RefSeq Synonyms
NM_013700, NM_001326594, BC066993,
Species
mouse
UniGene ID
Mm.3571
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.