mouse TCEA3 shRNA silencing Adenovirus

Name 
Ad-m-TCEA3-shRNA
Cat No 
shADV-273893
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Tcea3.

shADV-273893
Ad-m-TCEA3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Tcea3 (transcription elongation factor A (SII), 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
S-II; SII-K1
Description (Vector)
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Gene ID
21401
Gene Name (Vector)
transcription elongation factor A (SII), 3
Gene Symbol
Tcea3
ORF Size (aa)
1044
ORF Size (bp)
1044 bp
RefSeq ID
NM_011542
RefSeq Synonyms
NM_011542, BC010807,
Species
mouse
UniGene ID
Mm.112

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.