mouse SPAM1 shRNA silencing Adenovirus

Name 
Ad-m-SPAM1-shRNA
Cat No 
shADV-272933
Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Spam1.

shADV-272933
Ad-m-SPAM1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Spam1 (sperm adhesion molecule 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AV039194; Ph-20
Description (Vector)
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Gene ID
20690
Gene Name (Vector)
sperm adhesion molecule 1
Gene Symbol
Spam1
ORF Size (aa)
1539
ORF Size (bp)
1539 bp
RefSeq ID
NM_009241
RefSeq Synonyms
NM_009241, NM_001079875, BC148808, BC146388,
Species
mouse
UniGene ID
Mm.4688

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.